Date (Publication)

2018-10-02

Identifier

FR-330-715-368-00032-DATARMOR-BIOINFO_QL_TL_F2_2017

Identifier

DOI:10.12770/2567b981-0f7d-4215-8765-ee7258371af5

Credit

Chin-Long KY, Unité Ressources Marines en Polynésie

Thèmes Sextant

• /Milieu biologique/Bio-Informatique

GEMET - INSPIRE themes, version 1.0

• Habitats et biotopes

ODATIS aggregation parameters and Essential Variable names

• Bioinformatique

Use limitation

CC-BY (Creative Commons - Attribution)

Access constraints

License

Use constraints

License

Spatial representation type

Vector

Metadata language

Français

Character set

UTF8

Topic category

• Oceans

Unique resource identifier

EPSG:4326

Distribution format

• ( )

OnLine resource

/home/ref-bioinfo/ifremer/rmpf/QLTL_F2_2017/ ( NETWORK:LINK )

Hierarchy level

Dataset

Statement

In this study, we aimed to investigate differential gene expression among spat of the pearl oyster Pinctada margaritifera with distinct growth phenotypes. Two groups of P. margaritifera spat belonging to the same F2 cohort were selected. Larvae were settled in controlled hatchery tanks using mussel rope collectors. After 5.5 months of rearing, the largest (n = 10) and smallest (n = 10) individuals, representing the head and the tail of the size distribution were selected as "Fast-growing" (hereafter referred to as F) and "Slow-growing" (hereafter referred to as S) phenotypes. Oysters were then dissected and their whole fresh tissues individually collected and preserved in RNAlater®at -80°C until RNA extraction. Total RNA extraction was performed on the whole soft tissues of animals using TRIzol®reagent (Invitrogen), following manufacturer’s recommendations. RNA integrity was assessed on a Bioanalyzer 2100 (Agilent Technologies, USA). Total RNA was dried in RNA-stable solution (Thermo Fisher Scientific) following manufacturer’s instructions and shipped at room temperature to McGill sequencing platform services (Montreal, Canada). RNA-seq libraries were generated using an Illumina TruSeq RNA Sample preparation kit according to manufacturer’s instructions (Illumina). RNA libraries were multiplexed (n = 10 individual libraries per sequencing lane) and sequenced on an Illumina HiSeq 4000 to produce 100-bp paired-end reads.

File identifier

2567b981-0f7d-4215-8765-ee7258371af5 XML

Metadata language

Français

Character set

UTF8

Hierarchy level

Dataset

Date stamp

2024-02-02T10:31:14.856Z

Metadata standard name

ISO 19115:2003/19139 - SEXTANT

Metadata standard version

1.0