SARS-CoV-2 whole genome sequencing in wastewaters
WGS of SARS-CoV-2 by Oxford Nanopore Technology from raw wastewater samples collected in France, 2020-2021
Simple
- Date (Creation)
- 2021-01-01
- Date (Publication)
- 2022-02-02
- Identifier
- FR-330-715-368-00032-IFR_BIOINFO_SEQ_SARS_OBEPINE
- Identifier
- DOI:10.12770/90a96749-8fa1-467d-9af8-bd4ab1ae48bd
- Credit
- Ifremer - Laboratoire Sante Environnement Microbiologie
Author
Laboratoire de Microbiologie, LSEM, Ifremer.
-
Barbe Laure
Centre Atlantique - Rue de l'Ile d'Yeu - BP 21105 - 44311 Nantes Cedex 03
,
Nantes
,
France
02 40 37 43 33
- GEMET - INSPIRE themes, version 1.0
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- Habitats and biotopes
- Thèmes Sextant
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- /Biological Environment/Bioinformatics
- Access constraints
- License
- Use constraints
- License
- Spatial representation type
- Grid
- Metadata language
- English
- Character set
- UTF8
N
S
E
W
- Begin date
- 2021-01-01
- End date
- 2021-12-31
- Reference system identifier
- EPSG / WGS 84 (EPSG:4326) / 8.6
Spatial representation info
No information provided.
- Number of dimensions
- 0
- Cell geometry
- Area
- Distribution format
-
-
(
)
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(
)
- OnLine resource
- /home/ref-bioinfo/ifremer/obepine ( NETWORK:LINK )
- OnLine resource
-
Lien de téléchargement
(
WWW:DOWNLOAD-1.0-link--download
)
Lien de téléchargement
- OnLine resource
- Digital Object Identifier (DOI) ( WWW:LINK-1.0-http--metadata-URL )
- Hierarchy level
- Dataset
- Statement
- Raw wastewaters (WW) were collected weekly from two WW treatment plants in a city in western France. A subsample was ultracentrifuged, nucleic acids axtracted from the pellet, RNA reverse-transcribed and submitted to an optimized ARTIC400 V3 PCR. PCR products were purified, barcoded and adapter-ligated using Oxford Nanopore Technology kits, and libraries sequenced on an R9.4 flow cells using a Mk1c MinION. Results from 6 runs are shared as raw data (fast5 files) and based-called data using Guppy 4.3.4 (fastq files).
- File identifier
- 90a96749-8fa1-467d-9af8-bd4ab1ae48bd XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Date stamp
- 2023-07-06T11:56:49.926Z
- Metadata standard name
- ISO 19115:2003/19139 - SEXTANT
- Metadata standard version
- 1.0
Point of contact
IFREMER
-
Desdouits Marion
Station de Palavas - Chemin de Maguelone - 34250 Palavas les Flots
,
Palavas
,
France
04 67 13 04 14
Overviews
Spatial extent
N
S
E
W
Provided by
Associated resources
Not available