Date (Publication)

2024-10-31

Date (Creation)

2020-02-13

Identifier

FR-330-715-368-00032-IFR_BIOINFO_CRASSOSTREA_GIGAS_20240209_IHPE_EV_HEMOFIGHT

Credit

IHPE UMR5244

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Thèmes Sextant

• /Biological Environment/Bioinformatics

ODATIS aggregation parameters and Essential Variable names

• Bioinformatics

Use limitation

CC-BY (Creative Commons - Attribution)

Access constraints

Restricted

Use constraints

Copyright

Other constraints

Accès restreint jusqu'à la publication de l'article

Metadata language

English

Character set

UTF8

Topic category

• Environment

Reference system identifier

EPSG / WGS 84 (EPSG:4326) / 8.6

Distribution format

• ( )

OnLine resource

/home/ref-bioinfo-public/ifremer/ihpe/hemofight ( NETWORK:LINK )

OnLine resource

Lien de téléchargement ( WWW:DOWNLOAD-1.0-link--download )

Lien de téléchargement

OnLine resource

Publication ENA ( WWW:LINK-1.0-http--link )

OnLine resource

Digital Object Identifier (DOI) ( WWW:LINK-1.0-http--metadata-URL )

Hierarchy level

Dataset

Statement

The 10X Genomics protocol was followed to prepare gel in emulsion beads containing single cells, hydrogel beads, and reverse transcription reagents, perform barcoded cDNA synthesis, and generate sequencing libraries from pooled cDNAs. The concentration of single cell suspension was approximately 1000 cells / μL, and cells were loaded according to the 10X protocol to capture approximately 3000 cells per reaction. Library construction was performed using 10X reagents according to the manufacturer. Libraries (paired end reads 75 bp) were sequenced on an Illumina NovaSeq using two sequencing lane per sample.

File identifier

ee5fd99f-d88f-4c6c-9b77-bfe1c88ca86f XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Date stamp

2024-07-22T15:22:06.703Z

Metadata standard name

ISO 19115:2003/19139 - SEXTANT

Metadata standard version

1.0