Bioinformatics
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Fate and interaction between Microcystis and its microbiome in estuary.
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scRNA-seq reads from a Pacific oyster (Crassostrea gigas) hemocyte preparation. Hemocytes were isolated from a unique immunologically naive animal (Ifremer Standardized Animal, 18 months) and single-cell drop-seq technology was applied to 3,000 individual hemocytes.
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In European sea bass like in other animals, the tongue plays a fundamental role in the mechanics of food ingestion. It is composed from the surface in depth of mucosa, submucosa, musculature and fibro cartilaginous skeleton. The tunica mucosa exhibits a stratified epithelium interrupted by numerous teeth differently distributed that erupt more or less completely from the layers below. The European sea bass tongue is composed of canine-like teeth, surrounded by taste buds and numerous fungiform and conical papillae. The tongue beeing directly in contact with external environment, the success of the adaptation of fishes to different environments in the context of global change, depends oamong other on the modifications occurring on the tongue structures. The present study investigates the potential effect of ocean acidification on the lingual transcriptome.
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The present data set concerne metabarcoding raw reads produced using 4 different PCR targeting polymerase or capside coding region of the genoyupe I and II of norovirus. Test samples of norovirus with serial dilutions in pure water and after a bio-accumulation in oysters. Sequencing was made after VirCapSeq-VERT approach.
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Dart Seq data gathered on Blue Shark in the framework of the PSTBS-IO project supported by funding from FAO, CSIRO Oceans and Atmosphere, AZTI Tecnalia, Institut de recherche pour le développement (IRD), and Research Institute for Tuna Fisheries (RITF) and financial assistance of the European Union (GCP/INT/233/EC – Population structure of IOTC species in the Indian Ocean), and POPSIZE project supported by FEAMP (2014-2020 UE N°508/2014), and Institut français de recherche pour l'Exploitation de la mer (Ifremer).
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Study of the composition of the microbiota of healthy C.gigas oysters after injection of Cg-BigDef1 at T0, T6 and T24 by 16S metabarcoding analysis.
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16S rRNA amplicon metabarcoding from samples of the GHASS2 cruise (Romanian sector of the Black Sea, september 2021). Samples originate from marine sediment gravity coring samples as well as submarine samples from microbial mats and microbial reefs.
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This study aims to characterize the assemblage of a synthetic bacterial community when exposed to the exudates of Alexandrium minutum and Prymnesium parvum in presence and absence of microalgal cells. Both free-living and host-cell-attached bacterial communities were analyzed.
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DNA sequencing of Crassostrea gigas Pacific oyster spat infected in the wild with OsHV-1 virus in 4 French oyster basins (Marennes Oleron Bay, Arcachon bay, Rade de Brest and Thau lagoon).
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Metabarcoding data were produced based on samples gathered at Ifremer where the DNA was extracted; PCR libraries were built at Ifremer and Genseq; libraries were sequenced at Novogene. The data to download contain: 1/d emultiplexed raw data, 2/ metadata, and 3) Scripts to process data and taxonomically assign DNA sequences 4) Rmarkdown to analyze communities.