EMODnet Chemistry aims to provide access to marine chemistry data sets and derived data products concerning eutrophication, ocean acidification, contaminants and litter. The chosen parameters are relevant for the Marine Strategy Framework Directive (MSFD), in particular for descriptors 5, 8, 9 and 10. The datasets contain standardized, harmonized and validated data collections from seafloor litter. Datasets concerning seafloor litter data are loaded in a central database after a semi-automated validation phase. Once loaded, a data assessment is performed in order to check data consistency and potential errors are corrected thanks to a feedback loop with data originators. EMODnet seafloor litter data and database are hosted and maintained by ‘Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Division of Oceanography (OGS/NODC)’ from Italy. For seafloor litter, the harmonized datasets contain all unrestricted EMODnet Chemistry data on seafloor litter data. Data are formatted following Guidelines and forms for gathering marine litter data, which can be found at: https://dx.doi.org/10.6092/15c0d34c-a01a-4091-91ac-7c4f561ab508 The updated vocabularies of admitted values are available at: https://vocab.seadatanet.org/search https://vocab.ices.dk/ The harmonized datasets can be downloaded as EMODnet Sea-floor litter data format version 1.0, which is a csv file, tab separated values.

In October 2019 we chose 15 sites from the 2019 EVHOE survey for environmental DNA (eDNA) sampling. The French international EVHOE bottom trawl survey is carried out annually during autumn in the BoB to monitor demersal fish resources. At each site, we sampled seawater using Niskin bottles deployed with a circular rosette. There were nine bottles on the rosette, each of them able to hold ∼5 l of water. At each site, we first cleaned the circular rosette and bottles with freshwater, then lowered the rosette (with bottles open) to 5 m above the sea bottom, and finally closed the bottles remotely from the boat. The 45 l of sampled water was transferred to four disposable and sterilized plastic bags of 11.25 l each to perform the filtration on-board in a laboratory dedicated to the processing of eDNA samples. To speed up the filtration process, we used two identical filtration devices, each composed of an Athena® peristaltic pump (Proactive Environmental Products LLC, Bradenton, Florida, USA; nominal flow of 1.0 l min–1 ), a VigiDNA 0.20 μm filtration capsule (SPYGEN, le Bourget du Lac, France), and disposable sterile tubing. Each filtration device filtered the water contained in two plastic bags (22.5 l), which represent two replicates per sampling site. We followed a rigorous protocol to avoid contamination during fieldwork, using disposable gloves and single-use filtration equipment and plastic bags to process each water sample. At the end of each filtration, we emptied the water inside the capsule that we replaced by 80 ml of CL1 conservation buffer and stored the samples at room temperature following the specifications of the manufacturer (SPYGEN, Le Bourget du Lac, France). We processed the eDNA capsules at SPYGEN, following the protocol proposed by Polanco-Fernández et al., (2020). Half of the extracted DNA was processed by Sinsoma using newly developped ddPCR assays for European seabass (Dicentrachus labrax), European hake (Merluccius merluccius) and blackspot seabream (Pagellus bogaraveo).  The other half of the extracted DNA was analysed using metabarcoding with teleo primer. The raw metabarcoding data set is available at https://www.doi.org/10.16904/envidat.442 Bottom trawling using a GOV trawl was carried out before or after water sampling. The catch was sorted by species and catches in numbers and weight were recorded. No blackspot seabream individuals were caught.   Data content: * ddPCR/: contains the ddPCR counts and DNA concentrations for each sample and species. * SampleInfo/: contains the filter volume for each eDNA sample. * StationInfo/: contains metadata related to the data collected in the field for each filter. * Metabarcoding/: contains metabarcoding results for teleoprimer. * Trawldata/: contains catch data in numbers and weight (kg).      

EMODnet Chemistry aims to provide access to marine chemistry datasets and derived data products concerning eutrophication, acidity and contaminants. The importance of the selected substances and other parameters relates to the Marine Strategy Framework Directive (MSFD). This aggregated dataset contains all unrestricted EMODnet Chemistry data on eutrophication and acidity, and covers the Mediterranean Sea. Data were aggregated and quality controlled by the 'Hellenic Centre for Marine Research, Hellenic National Oceanographic Data Centre (HCMR/HNODC)' in Greece. ITS-90 water temperature and water body salinity variables have also been included ('as are') to complete the eutrophication and acidity data. If you use these variables for calculations, please refer to SeaDataNet for the quality flags: https://www.seadatanet.org/Products/Aggregated-datasets. Regional datasets concerning eutrophication and acidity are automatically harvested, and the resulting collections are aggregated and quality controlled using ODV Software and following a common methodology for all sea regions (https://doi.org/10.13120/8xm0-5m67 ) Parameter names are based on P35 vocabulary, which relates to EMODnet Chemistry aggregated parameter names and is available at: https://vocab.nerc.ac.uk/search_nvs/P35/. When not present in original data, water body nitrate plus nitrite was calculated by summing all nitrate and nitrite parameters. The same procedure was applied for water body dissolved inorganic nitrogen (DIN), which was calculated by summing all nitrate, nitrite, and ammonium parameters. Concentrations per unit mass were converted to a unit volume using a constant density of 1.025 kg/L. The aggregated dataset can also be downloaded as an ODV collection and spreadsheet, which is composed of a metadata header followed by tab separated values. This spreadsheet can be imported to ODV Software for visualisation (more information can be found at: https://www.seadatanet.org/Software/ODV ).

Seasonal climatology of Water body dissolved oxygen concentration for Loire river for the period 1950-2024 and for the following seasons: - winter: January-March, - spring: April-June, - summer: July-September, - autumn: October-December. Observation data span from 1950 to 2024. Depth levels (m): [0.0, 2.0, 4.0, 6.0, 8.0, 10.0, 15.0, 20.0, 25.0, 30.0, 35.0, 40.0, 45.0, 50.0, 60.0, 70.0, 80.0, 90.0, 100.0, 110.0, 120.0, 130.0]. Data sources: observational data from SeaDataNet/EMODNet Chemistry Data Network. Description of DIVAnd analysis: the computation was done with DIVAnd (Data-Interpolating Variational Analysis in n dimensions), version 2.7.12, using GEBCO 15 sec topography for the spatial connectivity of water masses. The horizontal resolution of the produced DIVAnd maps is 0.01 degrees. Horizontal correlation length is defined seasonally (in meters): 150000 (winter), 100000 (spring), 70000 (summer), 115000 (autumn). Vertical correlation length was optimized and vertically filtered and a seasonally-averaged profile was used (DIVAnd.fitvertlen). Signal-to-noise ratio was fixed to 1 for vertical profiles and 0.1 for time series to account for the redundancy in the time series observations. A logarithmic transformation (DIVAnd.loglin) was applied to the data prior to the analysis. Background field: the vertically-filtered data mean profile is substracted from the data. Detrending of data: no, advection constraint applied: no. Units: umol/l.

Moving 6-year analysis of Water body phosphate in the Mediterranean Sea for each season: - winter: January-March, - spring: April-June, - summer: July-September, - autumn: October-December. Every year of the time dimension corresponds to the 6-year centered average of the season. 6-years periods span from 1970-1975 until 2018-2023. Description of DIVA analysis: The computation was done with the DIVAnd (Data-Interpolating Variational Analysis in n dimensions), version 2.7.12, using GEBCO 30sec topography for the spatial connectivity of water masses. The horizontal resolution of the produced DIVAnd maps grids is dx=dy=0.125 degrees (around 13.5km and 10.9km accordingly). The vertical resolution is 20 depth levels: [0.,5.,10.,20.,30.,50.,75.,100.,125.,150.,200.,250.,300.,400.,500.,600.,700.,800.,900.,1000.]. The horizontal correlation length is 200km. The vertical correlation length (in meters) was set twices the vertical resolution: [10.,10.,20.,20.,40.,50.,50.,50.,50.,100.,100.,100.,200.,200.,200.,200.,200.,200.,200.,200.]. Duplicates check was performed using the following criteria for space and time: dlon=0.001deg., dlat=0.001deg., ddepth=1m, dtime=1hour, dvalue=0.1. The error variance (epsilon2) was set equal to 1 for profiles and 10 for time series to reduce the influence of close data near the coasts. An anamorphosis transformation was applied to the data (function DIVAnd.Anam.loglin) to avoid unrealistic negative values: threshold value=200. A background analysis field was used for all years (1970-2023) with correlation length equal to 600km and error variance (epsilon2) equal to 20. Quality control of the observations was applied using the interpolated field (QCMETHOD=3). Residuals (differences between the observations and the analysis (interpolated linearly to the location of the observations) were calculated. Observations with residuals outside the minimum and maximum values of the 99% quantile were discarded from the analysis. Originators of Italian data sets-List of contributors: - Brunetti Fabio (OGS) - Cardin Vanessa, Bensi Manuel doi:10.6092/36728450-4296-4e6a-967d-d5b6da55f306 - Cardin Vanessa, Bensi Manuel, Ursella Laura, Siena Giuseppe doi:10.6092/f8e6d18e-f877-4aa5-a983-a03b06ccb987 - Cataletto Bruno (OGS) - Cinzia Comici Cinzia (OGS) - Civitarese Giuseppe (OGS) - DeVittor Cinzia (OGS) - Giani Michele (OGS) - Kovacevic Vedrana (OGS) - Mosetti Renzo (OGS) - Solidoro C.,Beran A.,Cataletto B.,Celussi M.,Cibic T.,Comici C.,Del Negro P.,De Vittor C.,Minocci M.,Monti M.,Fabbro C.,Falconi C.,Franzo A.,Libralato S.,Lipizer M.,Negussanti J.S.,Russel H.,Valli G., doi:10.6092/e5518899-b914-43b0-8139-023718aa63f5 - Celio Massimo (ARPA FVG) - Malaguti Antonella (ENEA) - Fonda Umani Serena (UNITS) - Bignami Francesco (ISAC/CNR) - Boldrini Alfredo (ISMAR/CNR) - Marini Mauro (ISMAR/CNR) - Miserocchi Stefano (ISMAR/CNR) - Zaccone Renata (IAMC/CNR) - Lavezza, R., Dubroca, L. F. C., Ludicone, D., Kress, N., Herut, B., Civitarese, G., Cruzado, A., Lefèvre, D.,Souvermezoglou, E., Yilmaz, A., Tugrul, S., and Ribera d'Alcala, M.: Compilation of quality controlled nutrient profiles from the Mediterranean Sea, doi:10.1594/PANGAEA.771907, 2011.

This visualization product displays the single use plastics (SUP) related plastic items abundance of marine macro-litter (> 2.5cm) per beach per year from Marine Strategy Framework Directive (MSFD) monitoring surveys. EMODnet Chemistry included the collection of marine litter in its 3rd phase. Since the beginning of 2018, data of beach litter have been gathered and processed in the EMODnet Chemistry Marine Litter Database (MLDB). The harmonization of all the data has been the most challenging task considering the heterogeneity of the data sources, sampling protocols and reference lists used on a European scale. Preliminary processings were necessary to harmonize all the data: - Exclusion of OSPAR 1000 protocol: in order to follow the approach of OSPAR that it is not including these data anymore in the monitoring; - Selection of MSFD surveys only (exclusion of other monitoring, cleaning and research operations); - Exclusion of beaches without coordinates; - Selection of SUP related items only. The list of selected items is attached to this metadata. This list was created using EU Marine Beach Litter Baselines, the European Threshold Value for Macro Litter on Coastlines and the Joint list of litter categories for marine macro-litter monitoring from JRC (these three documents are attached to this metadata). The selection was adapted to the Joint list of litter categories SUP identification and therefore contains some differences with the selection made for previously published versions of this product; - Normalization of survey lengths to 100m & 1 survey / year: in some case, the survey length was not exactly 100m, so in order to be able to compare the abundance of litter from different beaches a normalization is applied using this formula: Number of SUP items of the survey (normalized by 100 m) = Number of SUP related items of the survey x (100 / survey length) Then, this normalized number of¨SUP related items is summed to obtain the total normalized number of SUP related items for each survey. Finally, the median abundance of SUP related items for each beach and year is calculated from these normalized abundances of SUP related items per survey. Sometimes the survey length was null or equal to 0. Assuming that the MSFD protocol has been applied, the length has been set at 100m in these cases. Percentiles 50, 75, 95 & 99 have been calculated taking into account SUP related plastic items from MSFD data for all years. More information is available in the attached documents. Warning: the absence of data on the map does not necessarily mean that they do not exist, but that no information has been entered in the Marine Litter Database for this area.

Moving 6-year analysis of Water body silicate in the NorthEast Atlantic for each season: - winter: January-March, - spring: April-June, - summer: July-September, - autumn: October-December. Every year of the time dimension corresponds to the 6-year centred average of each season. 6-year periods span from 1950/1955 until 2019/2024. Observation data span from 1950 to 2024. Depth levels (IODE standard depths): [0.0, 5.0, 10.0, 20.0, 30.0, 40.0, 50.0, 75.0, 100.0, 125.0, 150.0, 200.0, 250.0, 300.0, 400.0, 500.0, 600.0, 700.0, 800.0, 900.0, 1000.0, 1100.0, 1200.0, 1300.0, 1400.0, 1500.0, 1750.0, 2000.0]. Data sources: observational data from SeaDataNet/EMODNet Chemistry Data Network. Description of DIVAnd analysis: the computation was done with DIVAnd (Data-Interpolating Variational Analysis in n dimensions), version 2.7.12, using GEBCO 30 sec topography for the spatial connectivity of water masses. The horizontal resolution of the produced DIVAnd maps is 0.1 degrees. Horizontal correlation length varies from 300km in open sea regions to 50km at the coast. Vertical correlation length is defined as twice the vertical resolution. Signal-to-noise ratio was fixed to 1 for vertical profiles and 0.1 for time series to account for the redundancy in the time series observations. A logarithmic transformation (DIVAnd.loglin) was applied to the data prior to the analysis. Background field: a vertically-filtered profile of the seasonal data mean value (including all years) is substracted from the data. Detrending of data: no, advection constraint applied: no. Units: umol/l.

This visualization product displays the fishing & aquaculture related plastic items abundance of marine macro-litter (> 2.5cm) per beach per year from non-MSFD monitoring surveys, research & cleaning operations. EMODnet Chemistry included the collection of marine litter in its 3rd phase. Since the beginning of 2018, data of beach litter have been gathered and processed in the EMODnet Chemistry Marine Litter Database (MLDB). The harmonization of all the data has been the most challenging task considering the heterogeneity of the data sources, sampling protocols and reference lists used on a European scale. Preliminary processings were necessary to harmonize all the data: - Exclusion of OSPAR 1000 protocol: in order to follow the approach of OSPAR that it is not including these data anymore in the monitoring; - Selection of surveys from non-MSFD monitoring, cleaning and research operations; - Exclusion of beaches without coordinates; - Selection of fishing and aquaculture related plastic items only. The list of selected items is attached to this metadata. This list was created using EU Marine Beach Litter Baselines, the European Threshold Value for Macro Litter on Coastlines and the Joint list of litter categories for marine macro-litter monitoring from JRC (these three documents are attached to this metadata). The selection was adapted to the Joint list of litter categories fishing gears identification and therefore contains some differences with the selection made for previously published versions of this product; - Exclusion of surveys without associated length; - Normalization of survey lengths to 100m & 1 survey / year: in some case, the survey length was not 100m, so in order to be able to compare the abundance of litter from different beaches a normalization is applied using this formula: Number of fishing & aquaculture related plastic items of the survey (normalized by 100 m) = Number of fishing & aquaculture related items of the survey x (100 / survey length) Then, this normalized number of fishing & aquaculture related plastic items is summed to obtain the total normalized number of fishing & aquaculture related plastic items for each survey. Finally, the median abundance of fishing & aquaculture related plastic items for each beach and year is calculated from these normalized abundances of fishing & aquaculture related items per survey. Percentiles 50, 75, 95 & 99 have been calculated taking into account fishing & aquaculture related plastic items from other sources data for all years. More information is available in the attached documents. Warning: the absence of data on the map does not necessarily mean that they do not exist, but that no information has been entered in the Marine Litter Database for this area.

Sardine physiological measurments from september to november 2020

This visualization product displays the number of non-MSFD monitoring surveys, research & cleaning operations and the associated temporal coverage per beach. EMODnet Chemistry included the collection of marine litter in its 3rd phase. Since the beginning of 2018, data of beach litter have been gathered and processed in the EMODnet Chemistry Marine Litter Database (MLDB). The harmonization of all the data has been the most challenging task considering the heterogeneity of the data sources, sampling protocols and reference lists used on a European scale. Preliminary processings were necessary to harmonize all the data: - Exclusion of OSPAR 1000 protocol: in order to follow the approach of OSPAR that it is not including these data anymore in the monitoring; - Selection of surveys from non-MSFD monitoring, cleaning and research operations; - Exclusion of beaches without coordinates. More information is available in the attached documents. Warning: the absence of data on the map does not necessarily mean that they do not exist, but that no information has been entered in the Marine Litter Database for this area.