Dataset summary Plankton and detritus are essential components of the Earth’s oceans influencing biogeochemical cycles and carbon sequestration. Climate change impacts their composition and marine ecosystems as a whole. To improve our understanding of these changes, standardized observation methods and integrated global datasets are needed to enhance the accuracy of ecological and climate models. Here, we present a global dataset for plankton and detritus obtained by two versions of the Underwater Vision Profiler 5 (UVP5). This release contains the images classified in 33 homogenized categories, as well as the metadata associated with them, reaching 3,114 profiles and ca. 8 million objects acquired between 2008-2018 at global scale. The geographical distribution of the dataset is unbalanced, with the Equatorial region (30° S - 30° N) being the most represented, followed by the high latitudes in the northern hemisphere and lastly the high latitudes in the Southern Hemisphere. Detritus is the most abundant category in terms of concentration (90%) and biovolume (95%), although its classification in different morphotypes is still not well established. Copepoda was the most abundant taxa within the plankton, with Trichodesmium colonies being the second most abundant. The two versions of UVP5 (SD and HD) have different imagers, resulting in a different effective size range to analyse plankton and detritus from the images (HD objects >600 µm, SD objects >1 mm) and morphological properties (grey levels, etc.) presenting similar patterns, although the ranges may differ. A large number of images of plankton and detritus will be collected in the future by the UVP5, and the public availability of this dataset will help it being utilized as a training set for machine learning and being improved by the scientific community. This will reduce uncertainty by classifying previously unclassified objects and expand the classification categories, ultimately enhancing biodiversity quantification. Data tables The data set is organised according to: - samples : Underwater Vision Profiler 5 profiles, taken at a given point in space and time. - objects : individual UVP images, taken at a given depth along the each profile, on which various morphological features were measured and that where then classified taxonomically in EcoTaxa. samples and objects have unique identifiers. The sample_id is used to link the different tables of the data set together. All files are Tab separated values, UTF8 encoded, gzip compressed. samples.tsv.gz - sample_id    <int>    unique sample identifier - sample_name    <text>    original sample identifier - project    <text>    EcoPart project title - lat, lon    <float>    location [decimal degrees] - datetime    <text>    date and time of start of profile [ISO 8601: YYYY-MM-DDTHH:MM:SSZ] - pixel_size    <float>    size of one pixel [mm] - uvp_model    <text>    version of the UVP: SD: standard definition, ZD: zoomed, HD: high definition samples_volume.tsv.gz Along a profile, the UVP takes many images, each of a fixed volume. The profiles are cut into 5 m depth bins in which the number of images taken is recorded and hence the imaged volume is known. This is necessary to compute concentrations. - sample_id    <int>    unique sample identifier - mid_depth_bin    <float>    middle of the depth bin (2.5 = from 0 to 5 m depth) [m] - water_volume_imaged    <float>    volume imaged = number of full images × unit volume [L] objects.tsv.gz - object_id    <int>     unique object identifier - object_name    <text>     original object identifier - sample_id    <int>     unique sample identifier - depth    <float>    depth at which the image was taken [m] - mid_depth_bin    <float>    corresponding depth bin [m]; to match with samples_volumes - taxon    <text>     original taxonomic name as in EcoTaxa; is not consistent across projects - lineage    <text>     taxonomic lineage corresponding to that name - classif_author    <text>     unique, anonymised identifier of the user who performed this classification - classif_datetime    <text>     date and time at which the classification was - group    <text>     broader taxonomic name, for which the identification is consistent over the whole dataset - group_lineage    <text>     taxonomic lineage corresponding to this broader group - area_mm2    <float>    measurements on the object, in real worl units (i.e. comparable across the whole dataset) … - major_mm    <float> - area    <float>    measurements on the objet, in [pixels] and therefore not directly comparable among the different UVP models and units - mean    <float> … - skeleton_area    <float> properties_per_bin.tsv.gz The information above allows to compute concentrations, biovolumes, and average grey level within a given depth bin. The code to do so is in `summarise_objects_properties.R`. - sample_id    <int>     unique sample identifier - depth_range    <text>     range of depth over which the concentration/biovolume are computed: (start,end], in [m] where `(` means not including, `]` means including - group    <text>     broad taxonomic group - concentration    <float>    concentration [ind/L] - biovolume    <float>    biovolume [mm3/L] - avg_grey    <float>    average grey level of particles [no unit; 0 is black, 255 is white] ODV_biovolumes.txt, ODV_concentrations.txt, ODV_grey_levels.txt This is the same information as above, formatted in a way that Ocean Data View https://odv.awi.de can read. In ODV, go to Import > ODV Spreadsheet and accept all default choices. Images The images are provided in a separate, much larger, zip file. They are stored with the format `sample_id/object_id.jpg`, where `sample_id` and `object_id` are the integer identifiers used in the data tables above.

A world deep displacement dataset comprising more than 1600 000 Argo floats deep displacements, has been produced from the global Argo float database (GDAC). ANDRO dataset was completed over the period 2000-2009, then was partially but yearly updated since 2010. ANDRO actual contents and format is described in the user guide, which must be carefully read before using ANDRO (ANDRO format is also described in Ollitrault M. et al (2013)). One important feature of ANDRO is that the pressures measured during float drifts at depth, and suitably averaged are preserved in ANDRO (see Figure 2). To reach this goal, it was necessary to reprocess most of the Argo raw data, because of the many different decoding versions (roughly 100) not always applied by the DACs to the displacement data because they were mainly interested in the p,t,S profiles. The result of our work was the production of comprehensive files, named DEP (for déplacements in French), containing all the possibly retrievable float data. For detailed information and status of the last released ANDRO product, please visit the dedicated Argo France web page: https://www.umr-lops.fr/SNO-Argo/Products/ANDRO-Argo-floats-displacements-Atlas

French Research vessels have been collecting thermo-salinometer (TSG) data since the early 2000 in contribution to the GOSUD programme. The set of homogeneous instruments is permanently monitored and regularly calibrated. Water samples are taken on a daily basis by the crew and later analysed in the laboratory. We present here the delayed mode processing of the time series intiated in 2001 dataset and an overview of the resulting quality. The careful calibration and instrument maintenance, complemented with a rigorous adjustment on water samples lead to reach an accuracy of a few 10-² PSS in salinity or evenless. Global comparison with the ISAS13 ARGO gridded product shows an excellent agreement of the datasets. The SSS-Fresh dataset appears as highly valuable for the "calibration and validation" of the new satellite observations delivered by SMOS, Aquarius and SMAP.

LOCEAN has been in charge of collecting sea water for the analysis of water isotopes on a series of cruises or ships of opportunity mostly in the equatorial Atlantic, in the North Atlantic, in the southern Indian Ocean, in the southern Seas, Nordic Seas, and in the Arctic. The LOCEAN data set of the oxygen and hydrogen isotope (δ18O and δD) of marine water covers the period 1998 to 2019, but the effort is ongoing. Most data prior to 2010 (only δ18O) were analyzed using isotope ratio mass spectrometry (Isoprime IRMS) coupled with a Multiprep system (dual inlet method), whereas most data since 2010 (and a few earlier data) were obtained by cavity ring down spectrometry (CRDS) on a Picarro CRDS L2130-I, or less commonly on a Picarro CRDS L2120-I. Occasionally, some data were also run by Marion Benetti on an Isoprime IRMS coupled to a GasBench (dual inlet method) at the university of Iceland (Reykjavik). On the LOCEAN Picarro CRDS, most samples were initially analyzed after distillation, but since 2016, they have often been analyzed using a wire mesh to limit the spreading of sea salt in the vaporizer. Some of the samples on the CRDS were analyzed more than once on different days, when repeatability for the same sample was not sufficient or the daily run presented a too large drift. Accuracy is best when samples are distilled, and for δD are better on the Picarro CRDS L2130-I than on the Picarro CRDS L2120-I. Usually, we found that the reproducibility of the δ18O measurements is within ± 0.05 ‰ and of the δD measurements within ± 0.30 ‰, which should be considered an upper estimate of the error on the measurement on a Picarro CRDS. The water samples were kept in darkened glass bottles (20 to 50 ml) with special caps, and were often (but not always) taped afterwards. Once brought back in Paris, the samples were often stored in a cold room (with temperature close to 4°C), in particular if they were not analyzed within the next three months. There is however the possibility that some samples have breathed during storage. We found it happening on a number of samples, more commonly when they were stored for more than 5 years before being analyzed. We also used during one cruise bottles with not well-sealed caps (M/V Nuka Arctica in April 2019), which were analyzed within 3 months, but for which close to one third of the samples had breathed. We have retained those analyses, but added a flag ‘3’ meaning probably bad, at least on d-excess (outside of regions where sea ice forms or melts, for the analyses done on the Picarro CRDS, excessive evaporation is usually found with a d-excess criterium (which tends to be too low); for the IRMS analyses, it is mostly based when excessive scatter is found in the S- δ18O scatter plots or between successive data, in which case some outliers were flagged at ‘3’). In some cases when breathing happened, we found that d-excess can be used to produce a corrected estimate of δ18O and δD (Benetti et al., 2016). When this method was used a flag ‘1’ is added, indicating ‘probably good’ data, and should be thought as not as accurate as the data with no ‘correction’, which are flagged ‘2’ or ‘0’. We have adjusted data to be on an absolute fresh-water scale based on the study of Benetti et al. (2017), and on further tests with the different wire meshes used more recently. We have also checked the consistency of the runs in time, as there could have been changes in the internal standards used. On the Isoprime IRMS, it was mostly done using different batches of ‘Eau de Paris’ (EDP), whereas on the Picarro CRDS, we used three internal standards kept in metal tanks with a slight overpressure of dry air). The internal standards have been calibrated using VSMOW and GISP, and were also sent to other laboratories to evaluate whether they had drifted since the date of creation (as individual sub-standards have typically stored for more than 5-years). These comparisons are still not fully statisfactory to evaluate possible drifts in the sub-standards. Version V5 contains only one global file (ALL-Wisotopes-V5). However, up to version V4, individual files corresponded to regional subsets : - SO: Southern Ocean including cruise station and surface data mostly from 2017 in the Weddell Sea (WAPITI Cruise JR160004, DOI:10.17882/54012), as well as in the southern Ocean south of 20°S - SI: OISO cruise station and surface data in the southern Indian Ocean (since 1998) (DOI:10.18142/228) - EA: 20°N-20°S cruise station and surface data (since 2005), in particular in the equatorial Atlantic from French PIRATA (DOI:10.18142/14) and EGEE cruises (DOI:10.18142/95) - NA: 20°N-72°N station and surface data, mostly in the North Atlantic from Oceanographic cruises as well as from ships of opportunity (this includes in particular OVIDE cruise data since 2002 (DOI:10.17882/46448),  CATARINA, BOCATS1 and BOCATS2 (PID2019-104279GB-C21/AEI/10.13039/501100011033) cruises funded by the Spanish Research Agency, RREX2017 2017 cruise data (DOI:10.17600/17001400), SURATLANT data set since 2011 (DOI:10.17882/54517), Nuka Arctica and Tukuma Arctica data since 2012, STRASSE (DOI:10.17600/12040060) and MIDAS cruise data in 2012-2013, as well as surface data from various ships of opportunity since 2012) - NS: Nordic Sea data from cruises in 2002-2018 - AS: Arctic Ocean north of 72°N, in particular from two Tara cruises (in 2006-2008 and 2013) and expeditions since 2020 - PM: miscellaneous data in tropical Pacific, Indian Ocean, Mediterranean Sea and Black Sea In some regions, such as in the Indian Ocean, it is valuable to combine different subsets to have the full data distribution. The files are in csv format reported, and starting with version V1, it is reported as: - Cruise name, station id, bottle number, day, month, year, hour, minute, latitude, longitude, pressure (db), temperature (°C), it, salinity (pss-78), is, dissolved oxygen (micromol/kg), io2, δ18O, iO, d D, iD, d-excess, id, method type - Temperature is an in situ temperature - Salinity is a practical salinity it, is, io2, iO, iD, id are quality indices equal to: - 0 no quality check (but presumably good data) - 1 probably good data - 2 good data - 3 probably bad data - 4 certainly bad data - 9 missing data (and the missing data are reported with an unlikely missing value) The method type is 1 for IRMS measurements, 2 for CRDS measurement of a saline water sample, 3 for CRDS measurement of a distilled water sample.

EMODnet Chemistry aims to provide access to marine chemistry datasets and derived data products concerning eutrophication, acidity, contaminants and marine litter. The importance of the selected substances and other parameters relates to the Marine Strategy Framework Directive (MSFD). This aggregated dataset contains all unrestricted EMODnet Chemistry data on floating micro-litter. This dataset is the result of a validation and harmonisation process of the datasets concerning floating micro-litter present in EMODnet Chemistry. The datasets concerning micro-litter are automatically harvested and the resulting collections are harmonised and validated using ODV Software and following a common methodology for all sea regions. Parameter names are based on P01 vocabulary, which relates to BODC Parameter Usage Vocabulary and is available at: https://vocab.nerc.ac.uk/search_nvs/P01/ This process was performed by ‘Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Division of Oceanography (OGS/NODC)’ from Italy. Harmonisation means that: (1) unit conversion is carried out to express variables with a limited set of measurement units and (2) merging of variables described by different “local names”, but corresponding exactly to the same concepts in BODC P01 vocabulary. The harmonised dataset can be downloaded as ODV collection that can be opened with ODV software for visualization (More information can be found at: https://www.seadatanet.org/Software/ODV ). The same dataset is offered as spreadsheet (txt format, tab separated values) where the values of the categories for the following reported parameters (type, shape, size, color, transparency and material) have been uniformed as labelled in the SeaDataNet H01, H02, H03, H04, H05, H06 vocabularies (https://vocab.seadatanet.org/search ). This format is more adapted to worksheet applications (e.g. LibreOffice Calc).

The Arcachon bay is a meso- / macro-tidal (0.8 to 4.6 m), semi-enclosed lagoon of 180 km² located on the South-western coast of France. Three main water masses are described in this bay: (i) the external neritic waters (ENW) directly influenced by the adjacent oceanic waters, (ii) the intermediate neritic waters (ItNW) and (iii) the inner neritic waters (InNW) more influenced by the continental inputs. The watershed of the Arcachon bay, mainly covered by forests, has an area of 3500 km² and the bay is considered as poorly anthropised. It hosts the largest Zostera noltei seagrass meadow in western Europe and is an important site for oyster farming and Manilla clam production. Since 1997, Arcachon Bay waters are monitored for hydrological and bio-geochemical parameters by the “Environnements et Paléoenvironnements Océaniques et Continentaux” (EPOC) Research Unit of the University of Bordeaux-CNRS, first in one single station (Eyrac), then on 2 complementary sites since 2005 (Bouee13 and Comprian). The monitoring is carried out within the national framework of the “SOMLIT” (“Service d’Observation en Milieu Littoral”) which is a French multi-site monitoring network initiated in the mid-1990s. SOMLIT is based on a joint strategy for 19 sites belonging to 12 ecosystems that are distributed over the three maritime facades of mainland France, i.e. the English Channel, the Atlantic Ocean and the Mediterranean Sea. Sampling of surface water samples is performed fortnightly at high tide for a group of 15 parameters (temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonium, phosphate, silicate, suspended matter, chlorophyll a, concentrations and isotopic ratios of particulate organic carbon and nitrogen) and 8 flow cytometry biological variables of pico- and nanoplankton. Vertical profiles of multiparametric probes concerning 4 parameters (temperature, salinity, fluorescence, PAR) are also performed. Given the significant diversity of coastal ecosystems where SOMLIT’s stations are located, strict and joint guidelines with regards to sampling strategy, measurement methods and data qualification and storage are paramount in order to make FAIR data available to users. The whole data acquisition strategy is carried out within the framework of the SOMLIT quality system formalized in 2006-2007 by referring to the ISO 17025: 2017 standard “General requirements for the competence of testing and calibration laboratories”. Unified sampling and analysis protocols are based on recognized disciplinary standards and on the expertise of the research teams. The scientific objectives of SOMLIT are 1) to characterize the multi-decadal evolution of coastal ecosystems; 2) to determine the climatic and anthropogenic forcings and 3) to make data and logistical support available for research activities and other observation activities. SOMLIT is therefore a research tool providing large datasets that also serve as logistical support for related research actions (from seasonal to long-term studies). Two additional national networks operate at the same SOMLIT sites: “COAST-HF” network performs high-frequency measurements (automated in situ measurements every 10 to 20 minutes) and “PHYTOBS-network” provides microphytoplankton biodiversity data. SOMLIT, COAST-HF and PHYTOBS are elementary networks of the Research Infrastructure “Infrastructure Littorale et Côtière” (ILICO) and are National Observation Services (SNO) of the Institut National des Sciences de l'Univers (INSU).

This visualization product displays the cigarette related items abundance of marine macro-litter (> 2.5cm) per beach per year from non-MSFD monitoring surveys, research & cleaning operations without UNEP-MARLIN data. EMODnet Chemistry included the collection of marine litter in its 3rd phase. Since the beginning of 2018, data of beach litter have been gathered and processed in the EMODnet Chemistry Marine Litter Database (MLDB). The harmonization of all the data has been the most challenging task considering the heterogeneity of the data sources, sampling protocols and reference lists used on a European scale. Preliminary processings were necessary to harmonize all the data: - Exclusion of OSPAR 1000 protocol: in order to follow the approach of OSPAR that it is not including these data anymore in the monitoring; - Selection of surveys from non-MSFD monitoring, cleaning and research operations; - Exclusion of beaches without coordinates; - Selection of cigarette related items only. The list of selected items is attached to this metadata. This list was created using EU Marine Beach Litter Baselines, the European Threshold Value for Macro Litter on Coastlines and the Joint list of litter categories for marine macro-litter monitoring from JRC (these three documents are attached to this metadata); - Exclusion of surveys without associated length; - Exclusion of surveys referring to the UNEP-MARLIN list: the UNEP-MARLIN protocol differs from the other types of monitoring in that cigarette butts are surveyed in a 10m square. To avoid comparing abundances from very different protocols, the choice has been made to distinguish in two maps the cigarette related items results associated with the UNEP-MARLIN list from the others; - Normalization of survey lengths to 100m & 1 survey / year: in some case, the survey length was not 100m, so in order to be able to compare the abundance of litter from different beaches a normalization is applied using this formula: Number of cigarette related items of the survey (normalized by 100 m) = Number of cigarette related items of the survey x (100 / survey length) Then, this normalized number of cigarette related items is summed to obtain the total normalized number of cigarette related items for each survey. Finally, the median abundance of cigarette related items for each beach and year is calculated from these normalized abundances of cigarette related items per survey. Percentiles 50, 75, 95 & 99 have been calculated taking into account cigarette related items from other sources data (excluding UNEP-MARLIN protocol) for all years. More information is available in the attached documents. Warning: the absence of data on the map does not necessarily mean that they do not exist, but that no information has been entered in the Marine Litter Database for this area.

The Mediterranean Sea is a natural laboratory to address questions about the formation and evolution of continental margins and the relationship between surface and deep processes. Different regional to local events have influenced the Neogene stratigraphic evolution of the Valencia and Menorca basins. The evaporites deposited during the Messinian Salinity Crisis (MSC) strongly impact its sedimentary and geomorphological evolution. Here we present a compilation of the main regional seismic stratigraphic markers from the continental platform to the deep sea. We provide in xyz format (z in second twt) the original picking files, (not interpolated) and interpolated grid of: i) the top of the Mesozoic formation, the base of the Neogene formations including the early Miocene volcanic features, ii) the top Burdigalian, Langhian, and Serravallian seismic horizons, iii) the seismic horizons related to the Messinian Salinity Crisis, iv) the Pliocene and Pleistocene seismic horizons v) the depth of the Seafloor. The available reflection seismic dataset results from the compilation and processing of vintage seismic profiles of previous works and from the Instituto Geologico y Minero de Espana (IGME). This compilation is currently the first available in literature and provides a useful contribution to the scientific community working on sedimentary, tectonics and geodynamics within the Western Mediterranean basins.

EMODnet Chemistry aims to provide access to marine chemistry data sets and derived data products concerning eutrophication, ocean acidification, contaminants and litter. The chosen parameters are relevant for the Marine Strategy Framework Directive (MSFD), in particular for descriptors 5, 8, 9 and 10. The datasets contain standardized, harmonized and validated data collections from seafloor litter. Datasets concerning seafloor litter data are loaded in a central database after a semi-automated validation phase. Once loaded, a data assessment is performed in order to check data consistency and potential errors are corrected thanks to a feedback loop with data originators. EMODnet seafloor litter data and database are hosted and maintained by ‘Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Division of Oceanography (OGS/NODC)’ from Italy. For seafloor litter, the harmonized datasets contain all unrestricted EMODnet Chemistry data on seafloor litter data. Data are formatted following Guidelines and forms for gathering marine litter data, which can be found at: https://dx.doi.org/10.6092/15c0d34c-a01a-4091-91ac-7c4f561ab508 The updated vocabularies of admitted values are available at: https://vocab.seadatanet.org/search https://vocab.ices.dk/ The harmonized datasets can be downloaded as EMODnet Sea-floor litter data format version 1.0, which is a csv file, tab separated values.

In October 2019 we chose 15 sites from the 2019 EVHOE survey for environmental DNA (eDNA) sampling. The French international EVHOE bottom trawl survey is carried out annually during autumn in the BoB to monitor demersal fish resources. At each site, we sampled seawater using Niskin bottles deployed with a circular rosette. There were nine bottles on the rosette, each of them able to hold ∼5 l of water. At each site, we first cleaned the circular rosette and bottles with freshwater, then lowered the rosette (with bottles open) to 5 m above the sea bottom, and finally closed the bottles remotely from the boat. The 45 l of sampled water was transferred to four disposable and sterilized plastic bags of 11.25 l each to perform the filtration on-board in a laboratory dedicated to the processing of eDNA samples. To speed up the filtration process, we used two identical filtration devices, each composed of an Athena® peristaltic pump (Proactive Environmental Products LLC, Bradenton, Florida, USA; nominal flow of 1.0 l min–1 ), a VigiDNA 0.20 μm filtration capsule (SPYGEN, le Bourget du Lac, France), and disposable sterile tubing. Each filtration device filtered the water contained in two plastic bags (22.5 l), which represent two replicates per sampling site. We followed a rigorous protocol to avoid contamination during fieldwork, using disposable gloves and single-use filtration equipment and plastic bags to process each water sample. At the end of each filtration, we emptied the water inside the capsule that we replaced by 80 ml of CL1 conservation buffer and stored the samples at room temperature following the specifications of the manufacturer (SPYGEN, Le Bourget du Lac, France). We processed the eDNA capsules at SPYGEN, following the protocol proposed by Polanco-Fernández et al., (2020). Half of the extracted DNA was processed by Sinsoma using newly developped ddPCR assays for European seabass (Dicentrachus labrax), European hake (Merluccius merluccius) and blackspot seabream (Pagellus bogaraveo).  The other half of the extracted DNA was analysed using metabarcoding with teleo primer. The raw metabarcoding data set is available at https://www.doi.org/10.16904/envidat.442 Bottom trawling using a GOV trawl was carried out before or after water sampling. The catch was sorted by species and catches in numbers and weight were recorded. No blackspot seabream individuals were caught.   Data content: * ddPCR/: contains the ddPCR counts and DNA concentrations for each sample and species. * SampleInfo/: contains the filter volume for each eDNA sample. * StationInfo/: contains metadata related to the data collected in the field for each filter. * Metabarcoding/: contains metabarcoding results for teleoprimer. * Trawldata/: contains catch data in numbers and weight (kg).