EMODnet Chemistry aims to provide access to marine chemistry datasets and derived data products concerning eutrophication, acidity and contaminants. The importance of the selected substances and other parameters relates to the Marine Strategy Framework Directive (MSFD). This aggregated dataset contains all unrestricted EMODnet Chemistry data on eutrophication and acidity, and covers the Mediterranean Sea. Data were aggregated and quality controlled by the 'Hellenic Centre for Marine Research, Hellenic National Oceanographic Data Centre (HCMR/HNODC)' in Greece. ITS-90 water temperature and water body salinity variables have also been included ('as are') to complete the eutrophication and acidity data. If you use these variables for calculations, please refer to SeaDataNet for the quality flags: https://www.seadatanet.org/Products/Aggregated-datasets. Regional datasets concerning eutrophication and acidity are automatically harvested, and the resulting collections are aggregated and quality controlled using ODV Software and following a common methodology for all sea regions (https://doi.org/10.13120/8xm0-5m67 ) Parameter names are based on P35 vocabulary, which relates to EMODnet Chemistry aggregated parameter names and is available at: https://vocab.nerc.ac.uk/search_nvs/P35/. When not present in original data, water body nitrate plus nitrite was calculated by summing all nitrate and nitrite parameters. The same procedure was applied for water body dissolved inorganic nitrogen (DIN), which was calculated by summing all nitrate, nitrite, and ammonium parameters. Concentrations per unit mass were converted to a unit volume using a constant density of 1.025 kg/L. The aggregated dataset can also be downloaded as an ODV collection and spreadsheet, which is composed of a metadata header followed by tab separated values. This spreadsheet can be imported to ODV Software for visualisation (more information can be found at: https://www.seadatanet.org/Software/ODV ).

The SOMLIT-Antioche observation station, located at 5 nautical miles from Chef de Baie harbor (La Rochelle) is part of the French monitoring network SOMLIT (https://www.somlit.fr/), accredited by the INSU-CNRS as a national Earth Science Observatory (Service National d’Observation : SNO), which comprises 12 observation stations distributed throughout France in coastal locations. It aims to detect long-term changes  of these ecosystems under both natural and anthropogenic forcings. SOMLIT is part of the national research infrastructure for coastal ocean observation ILICO (https://www.ir-ilico.fr/?PagePrincipale&lang=en). The SOMLIT-Antioche station (46.0842 °N, 1.30833 °W) is located in the north-eastern part of the Bay of Biscay, halfway between the islands of Ré and Oléron, at the centre of what is commonly known as the Pertuis Charentais area, which correspond to a semi-enclosed shallow basin and includes four islands (Ré, Oléron, Aix and Madame) and three Pertuis (i.e., detroit) (Breton, Antioche and Maumusson). This 40m-deep site, with muddy to sandy marine bottoms, is submitted to a macro-tidal regime and is largely open to the prevailing westerly swells. It remains under a dominant oceanic/neritic influence, even though its winter/spring hydrological context is influenced by the diluted plumes of the Charente, Gironde and Loire rivers, but not by those of too small estuaries (Lay, Seudre and Sèvre Niortaise). SOMLIT-Antioche hydrological monitoring has been carried out by the LIENSs/OASU laboratory on a fortnightly basis since June 2011. Surface water samples are collected  at high-tide during intermediate tides (70 ± 10 in SHOM units) on board the research  vessel ‘L’Estran’ owned by La Rochelle University. Samples are analyzed for more than 16 core parameters: temperature, salinity, dissolved oxygen, pH, ammonia, nitrates, nitrites, phosphates, silicates, suspended matter, particulate organic carbone, particulate organic nitrogen, chlorophyll, delta15N, delta13C; pico- and nano- plankton. Measurements are carried out in accordance with the ISO/IEC 17025:2017 standard. Simultaneous monitoring of the micro-phytoplankton community (since 2013, SNO PHYTOBS: https://www.phytobs.fr/en) and monitoring of prokaryotic communities (Bacteria and Archaea) are also carried out on a monthly basis. Since 2019, seasonal observations of benthic invertebrate communities (SNO BenthObs : https://www.benthobs.fr/) have also been carried out. This monitoring is complementary to that carried out at hydrological stations in the pre-existing REPHY and DCE networks, some of which are located near marine farming areas (oyster and mussel farms).

EMODnet Chemistry aims to provide access to marine chemistry data sets and derived data products concerning eutrophication, ocean acidification, contaminants and litter. The chosen parameters are relevant for the Marine Strategy Framework Directive (MSFD), in particular for descriptors 5, 8, 9 and 10. The dataset contains standardized, harmonized and validated data collections from beach litter (monitoring and other sources). Datasets concerning beach and seafloor litter data are loaded in a central database after a semi-automated validation phase. Once loaded, a data assessment is performed in order to check data consistency and potential errors are corrected thanks to a feedback loop with data originators. For beach litter, the harmonized datasets contain all unrestricted EMODnet Chemistry data on beach litter, including monitoring data, data from cleaning surveys and data from research. A relevant part of the monitoring data has been considered for assessment purposes by the European institutions and therefore is tagged as MSFD_monitoring. EMODnet beach litter data and databases are hosted and maintained by 'Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Division of Oceanography (OGS/NODC)' from Italy. Data are formatted following Guidelines and forms for gathering marine litter data, which can be found at: https://doi.org/10.6092/15c0d34c-a01a-4091-91ac-7c4f561ab508 The updated vocabularies of admitted values are available at: https://nodc.ogs.it/marinelitter/vocab The European harmonized dataset is provided as csv format in two files, one containing the survey data and one containing the survey metadata. The dataset is available through direct download or through ERDDAP.

Moving 6-year analysis and visualization of Water body phosphate in the North Sea. Four seasons (December-February, March-May, June-August, September-November). Data Sources: observational data from SeaDataNet/EMODnet Chemistry Data Network. Description of DIVA analysis: Geostatistical data analysis by DIVAnd (Data-Interpolating Variational Analysis) tool, version 2.7.12. results were subjected to the minfield option in DIVAnd to avoid negative/underestimated values in the interpolated results; error threshold masks L1 (0.3) and L2 (0.5) are included as well as the unmasked field. The depth dimension allows visualizing the gridded field at various depths.

Moving 6-year analysis of Water body phosphate in the Mediterranean Sea for each season: - winter: January-March, - spring: April-June, - summer: July-September, - autumn: October-December. Every year of the time dimension corresponds to the 6-year centered average of the season. 6-years periods span from 1970-1975 until 2018-2023. Description of DIVA analysis: The computation was done with the DIVAnd (Data-Interpolating Variational Analysis in n dimensions), version 2.7.12, using GEBCO 30sec topography for the spatial connectivity of water masses. The horizontal resolution of the produced DIVAnd maps grids is dx=dy=0.125 degrees (around 13.5km and 10.9km accordingly). The vertical resolution is 20 depth levels: [0.,5.,10.,20.,30.,50.,75.,100.,125.,150.,200.,250.,300.,400.,500.,600.,700.,800.,900.,1000.]. The horizontal correlation length is 200km. The vertical correlation length (in meters) was set twices the vertical resolution: [10.,10.,20.,20.,40.,50.,50.,50.,50.,100.,100.,100.,200.,200.,200.,200.,200.,200.,200.,200.]. Duplicates check was performed using the following criteria for space and time: dlon=0.001deg., dlat=0.001deg., ddepth=1m, dtime=1hour, dvalue=0.1. The error variance (epsilon2) was set equal to 1 for profiles and 10 for time series to reduce the influence of close data near the coasts. An anamorphosis transformation was applied to the data (function DIVAnd.Anam.loglin) to avoid unrealistic negative values: threshold value=200. A background analysis field was used for all years (1970-2023) with correlation length equal to 600km and error variance (epsilon2) equal to 20. Quality control of the observations was applied using the interpolated field (QCMETHOD=3). Residuals (differences between the observations and the analysis (interpolated linearly to the location of the observations) were calculated. Observations with residuals outside the minimum and maximum values of the 99% quantile were discarded from the analysis. Originators of Italian data sets-List of contributors: - Brunetti Fabio (OGS) - Cardin Vanessa, Bensi Manuel doi:10.6092/36728450-4296-4e6a-967d-d5b6da55f306 - Cardin Vanessa, Bensi Manuel, Ursella Laura, Siena Giuseppe doi:10.6092/f8e6d18e-f877-4aa5-a983-a03b06ccb987 - Cataletto Bruno (OGS) - Cinzia Comici Cinzia (OGS) - Civitarese Giuseppe (OGS) - DeVittor Cinzia (OGS) - Giani Michele (OGS) - Kovacevic Vedrana (OGS) - Mosetti Renzo (OGS) - Solidoro C.,Beran A.,Cataletto B.,Celussi M.,Cibic T.,Comici C.,Del Negro P.,De Vittor C.,Minocci M.,Monti M.,Fabbro C.,Falconi C.,Franzo A.,Libralato S.,Lipizer M.,Negussanti J.S.,Russel H.,Valli G., doi:10.6092/e5518899-b914-43b0-8139-023718aa63f5 - Celio Massimo (ARPA FVG) - Malaguti Antonella (ENEA) - Fonda Umani Serena (UNITS) - Bignami Francesco (ISAC/CNR) - Boldrini Alfredo (ISMAR/CNR) - Marini Mauro (ISMAR/CNR) - Miserocchi Stefano (ISMAR/CNR) - Zaccone Renata (IAMC/CNR) - Lavezza, R., Dubroca, L. F. C., Ludicone, D., Kress, N., Herut, B., Civitarese, G., Cruzado, A., Lefèvre, D.,Souvermezoglou, E., Yilmaz, A., Tugrul, S., and Ribera d'Alcala, M.: Compilation of quality controlled nutrient profiles from the Mediterranean Sea, doi:10.1594/PANGAEA.771907, 2011.

In order to better characterize the population structure of common dolphins (Delphinus delphis) in the Bay of Biscay, a single digest RADseq (SbfI enzyme) protocol was used to obtain paired-end, 150bp NGS sequences on the Illumina NovaSeq 6000 sequencing platform. D. delphis samples from the Western North Atlantic, and samples from three other delphinid species were included as outgroups.

EMODnet Chemistry aims to provide access to marine chemistry datasets and derived data products concerning eutrophication, acidity and contaminants. The importance of the selected substances and other parameters relates to the Marine Strategy Framework Directive (MSFD). This aggregated dataset contains all unrestricted EMODnet Chemistry data on eutrophication and acidity, and covers the Greater North Sea and Celtic Seas. Data were aggregated and quality controlled by 'Aarhus University, Department of Bioscience, Marine Ecology Roskilde' in Denmark. ITS-90 water temperature and water body salinity variables have also been included ('as are') to complete the eutrophication and acidity data. If you use these variables for calculations, please refer to SeaDataNet for the quality flags: https://www.seadatanet.org/Products/Aggregated-datasets . Regional datasets concerning eutrophication and acidity are automatically harvested, and the resulting collections are aggregated and quality controlled using ODV Software and following a common methodology for all sea regions ( https://doi.org/10.13120/8xm0-5m67 ). Parameter names are based on P35 vocabulary, which relates to EMODnet Chemistry aggregated parameter names and is available at: https://vocab.nerc.ac.uk/search_nvs/P35/ . When not present in original data, water body nitrate plus nitrite was calculated by summing all nitrate and nitrite parameters. The same procedure was applied for water body dissolved inorganic nitrogen (DIN), which was calculated by summing all nitrate, nitrite, and ammonium parameters. Concentrations per unit mass were converted to a unit volume using a constant density of 1.025 kg/L. The aggregated dataset can also be downloaded as an ODV collection and spreadsheet, which is composed of a metadata header followed by tab separated values. This spreadsheet can be imported to ODV Software for visualisation (more information can be found at: https://www.seadatanet.org/Software/ODV ).

In October 2019 we chose 15 sites from the 2019 EVHOE survey for environmental DNA (eDNA) sampling. The French international EVHOE bottom trawl survey is carried out annually during autumn in the BoB to monitor demersal fish resources. At each site, we sampled seawater using Niskin bottles deployed with a circular rosette. There were nine bottles on the rosette, each of them able to hold ∼5 l of water. At each site, we first cleaned the circular rosette and bottles with freshwater, then lowered the rosette (with bottles open) to 5 m above the sea bottom, and finally closed the bottles remotely from the boat. The 45 l of sampled water was transferred to four disposable and sterilized plastic bags of 11.25 l each to perform the filtration on-board in a laboratory dedicated to the processing of eDNA samples. To speed up the filtration process, we used two identical filtration devices, each composed of an Athena® peristaltic pump (Proactive Environmental Products LLC, Bradenton, Florida, USA; nominal flow of 1.0 l min–1 ), a VigiDNA 0.20 μm filtration capsule (SPYGEN, le Bourget du Lac, France), and disposable sterile tubing. Each filtration device filtered the water contained in two plastic bags (22.5 l), which represent two replicates per sampling site. We followed a rigorous protocol to avoid contamination during fieldwork, using disposable gloves and single-use filtration equipment and plastic bags to process each water sample. At the end of each filtration, we emptied the water inside the capsule that we replaced by 80 ml of CL1 conservation buffer and stored the samples at room temperature following the specifications of the manufacturer (SPYGEN, Le Bourget du Lac, France). We processed the eDNA capsules at SPYGEN, following the protocol proposed by Polanco-Fernández et al., (2020). Half of the extracted DNA was processed by Sinsoma using newly developped ddPCR assays for European seabass (Dicentrachus labrax), European hake (Merluccius merluccius) and blackspot seabream (Pagellus bogaraveo).  The other half of the extracted DNA was analysed using metabarcoding with teleo primer. The raw metabarcoding data set is available at https://www.doi.org/10.16904/envidat.442 Bottom trawling using a GOV trawl was carried out before or after water sampling. The catch was sorted by species and catches in numbers and weight were recorded. No blackspot seabream individuals were caught.   Data content: * ddPCR/: contains the ddPCR counts and DNA concentrations for each sample and species. * SampleInfo/: contains the filter volume for each eDNA sample. * StationInfo/: contains metadata related to the data collected in the field for each filter. * Metabarcoding/: contains metabarcoding results for teleoprimer. * Trawldata/: contains catch data in numbers and weight (kg).      

Observations of Sea surface temperature and salinity are now obtained from voluntary sailing ships using medium or small size sensors. They complement the networks installed on research vessels or commercial ships. The delayed mode dataset proposed here is upgraded annually as a contribution to GOSUD (http://www.gosud.org )

The present repository makes available the model, material and outputs of the ISIS-Fish modeling work showcased in the peer-reviewed scientific article by Bastardie et al. 2025. As part of the SEAwise research project (seawiseproject.org), we used an ISIS-Fish database (Mahevas et al 2003, Pelletier et al. 2009, isis-fish.org) previously developed within the MACCO project which describes the mixed demersal fishery in the Bay of Biscay. For this application, the spatial extent of the fishery is the Bay of Biscay, defined here by ICES divisions 8a, 8b and 8d and the resolution chosen is 1/16 ICES statistical rectangle. The biological module (Vajas et al. 2024) includes 7 species of economic interest in the mixed demersal fishery: European hake (Merluccius merluccius), common sole (Solea solea), Norway lobster (Nephrops norvegicus), megrim (Lepidorhombus whiffiagonis), anglerfish (Lophius piscatorius) and two ray species (Raja clavata, Leucoraja naevus). The fishing activities module (Mahevas et al. 2024) is made up of 41 demersal fleets (including all French vessels < 12 meters and > 12 meters fishing in this area, Spanich, UK and Belgium fleets) and 431 métiers (combination of a gear, location and mix of target species) catching these 7 species, as target or bycatch. Monthly effort of a fleet distributes among the possible métiers (those historically practiced). The biological and fishing activity modules are identical to the published version. The original model used here has been calibrated on historical catch data 2015-2018 by tuning accessibility and catchability parameters. In the present application the Bay of Biscay model is used to investigate the spatial- and effort- based fisheries management strategies. Consistently with for a task of the SEAwise project (Bastardie et al. 2024) simulations were conducted from 2021 onwards, projecting the effect of an implementation of 3 different closures from 2022 to 2050, under current fishing effort conditions or in a context of fishing effort reduction. Outcomes of these simulations are averaged over short/medium (10 year horizon) and long-term period (20 year horizon). The data project includes: 1) the database including the biological module and fishing activity module; 2) 8 .properties files, each corresponding to one combination of management measure and closure, to restore the simulations parameters in the ISIS-Fish interface and reproduce the simulation runs; 3) the .java scripts to force effort dynamics and simulate spatio-temporal closures, as well as generate the main output files - they will be called by the ISIS-Fish software once the simulations restored 4) the .rds containing the main outputs of the simulations and the associated .html document displaying the R code to compute the indices of interest at different levels of aggregation and reproduce the figures in Bastardie et al. 2025. All files are provided in the Zip. Associated with this material, a study summary and a readme .docx are provided. The first one provides context on the present work and describes the model and simulations' design. The second provides guidelines to reproduce the simulations and their derived outcomes from the data project material made available in this repository. They are both directly downloadable from this repository and are also copied to the zipped folder containing the data project. All the data are reproducible using isis-fish-4.4.8.1 (isis-fish.org; available at forge.codelutin.com) and R 4.2.0.