In October 2019 we chose 15 sites from the 2019 EVHOE survey for environmental DNA (eDNA) sampling. The French international EVHOE bottom trawl survey is carried out annually during autumn in the BoB to monitor demersal fish resources. At each site, we sampled seawater using Niskin bottles deployed with a circular rosette. There were nine bottles on the rosette, each of them able to hold ∼5 l of water. At each site, we first cleaned the circular rosette and bottles with freshwater, then lowered the rosette (with bottles open) to 5 m above the sea bottom, and finally closed the bottles remotely from the boat. The 45 l of sampled water was transferred to four disposable and sterilized plastic bags of 11.25 l each to perform the filtration on-board in a laboratory dedicated to the processing of eDNA samples. To speed up the filtration process, we used two identical filtration devices, each composed of an Athena® peristaltic pump (Proactive Environmental Products LLC, Bradenton, Florida, USA; nominal flow of 1.0 l min–1 ), a VigiDNA 0.20 μm filtration capsule (SPYGEN, le Bourget du Lac, France), and disposable sterile tubing. Each filtration device filtered the water contained in two plastic bags (22.5 l), which represent two replicates per sampling site. We followed a rigorous protocol to avoid contamination during fieldwork, using disposable gloves and single-use filtration equipment and plastic bags to process each water sample. At the end of each filtration, we emptied the water inside the capsule that we replaced by 80 ml of CL1 conservation buffer and stored the samples at room temperature following the specifications of the manufacturer (SPYGEN, Le Bourget du Lac, France). We processed the eDNA capsules at SPYGEN, following the protocol proposed by Polanco-Fernández et al., (2020). Half of the extracted DNA was processed by Sinsoma using newly developped ddPCR assays for European seabass (Dicentrachus labrax), European hake (Merluccius merluccius) and blackspot seabream (Pagellus bogaraveo).  The other half of the extracted DNA was analysed using metabarcoding with teleo primer. The raw metabarcoding data set is available at https://www.doi.org/10.16904/envidat.442 Bottom trawling using a GOV trawl was carried out before or after water sampling. The catch was sorted by species and catches in numbers and weight were recorded. No blackspot seabream individuals were caught.   Data content: * ddPCR/: contains the ddPCR counts and DNA concentrations for each sample and species. * SampleInfo/: contains the filter volume for each eDNA sample. * StationInfo/: contains metadata related to the data collected in the field for each filter. * Metabarcoding/: contains metabarcoding results for teleoprimer. * Trawldata/: contains catch data in numbers and weight (kg).      

210Pb, 226Ra and 137Cs were measured by non-destructive gamma spectrometry on marine sediment cores, collected during RIKEAU 2002 cruise on board r/v Thalia, on the shelf of the Bay of Biscay

Understanding the spatial and temporal preferences of toxic phytoplankton species is of paramount importance in managing and predicting harmful events in aquatic ecosystems. In this study we address the realised niche of the species Alexandrium minutum, Pseudo-nitzschia fraudulenta and P. australis. We used them to highlight distribution patterns at different scales and determine possible drivers. To achieve this, we have developed original procedures coupling niche theory and habitat suitability modelling using abundance data in four consecutive steps: 1) Estimate the realised niche applying kernel functions. 2) Assess differences between the species’ niche as a whole and at the local level. 3) Develop habitat and temporal suitability models using niche overlap procedures. 4) Explore species temporal and spatial distributions to highlight possible drivers. Data used are species abundance and environmental variables collected over 27 years (1988-2014) and include 139 coastal water sampling sites along the French Atlantic coast. Results show that A. minutum and P. australis niches are very different, although both species have preference for warmer months. They both respond to decadal summer NAO but in the opposite way. P. fraudulenta realised niche lies in between the two other species niches. It also prefers warmer months but does not respond to decadal summer NAO. The Brittany peninsula is now classified as an area of prevalence for the three species. The methodology used here will allow to anticipate species distribution in the event of future environmental challenges resulting from climate change scenarios.

Three saltmarshes, Aiguillon, Brouage, Fier d'Ars, located in the Pertuis-Charentais Sea along the south-west coast of France, were studied to evaluate their sediment and mass accumulation rates (SAR; MAR) based on 210Pb and 137Cs profiles in sediments. Coastal saltmarshes play indeed an essential role in providing services such as coastal protection and supporting biodiversity. Saltmarshes are also critical environments for the accumulation of sedimentary organic carbon (blue carbon). However, the number of studies on saltmarshes remains underrepresented compared to studies on mangroves and seagrass. This work is a contribution to the effort to document sediment and mass accumulation rates of saltmarshes.A total of 16 1m sediment cores were collected in the three saltmarshes (Aiguillon, Brouage, Fier d'Ars) in 2021 and 2022 using an Eijkelkamp stainless steel peat sampler. Each sediment core was sampled every 1 cm from the top to the bottom of the core. The sediment layers were used to determine dry bulk density and selected radioisotope activities (210Pb, 226Ra, 137Cs, 228Th, 137Cs). Combining excess 210Pb and 137Cs has allowed to establish a reliable chronology of sediment deposition on a multidecadal timescale.

Marine protected areas (MPAs) provide biodiversity conservation benefits in a range of marine habitats. Many protected areas are established and governed through top-down or shared governance arrangements, yet little is known about how these governance strategies compare in terms of the protection benefits they provide to MPAs globally. Using an extensive data set of MPA conditions, we developed a set of Bayesian hierarchical models to understand the role of shared governance versus federal governance on reef fish biomass from 218 global MPAs. We find greater reef fish biomass benefits in MPAs with shared governance than with top-down, or federal arrangements. We also find greater benefits in older MPAs and MPAs farther away from shore. Our results highlight the fundamental importance of multi-stakeholder participation for improving conservation outcomes, representing an important conservation opportunity for new or existing MPAs. 

The willingness to pay (WTP) of people to protect animal populations can be used as a tool for these populations’ conservation. The WTP reflects the non-use value of animals, which can be significant for charismatic species. This value can be used as an economic criterion for decision-makers in order to recommend protective measures. The definition of the WTP to protect a species is challenging, as valuation methods are time-consuming and expensive. To overcome these limitations, we built a benefit transfer function based on 112 valuation studies and apply it to 440 Mediterranean marine species. We extracted these species from the IUCN database and retrieved some required parameters from, amongst others, the FishBase database. Marine mammals appear to have the highest WTP value followed in order by sea turtles, sharks and rays, and ray-finned fishes. Commercial fish species appear to have the highest values amongst the fish class.

Aires géographiques des appellations d'origine contrôlées (AOC)/protégées (AOP). Le fichier liste pour chaque commune, identifiée par son département, son nom et son code INSEE, les aires géographiques des appellations AOC/AOP qui se situent sur la commune

Worldwide, shellfish aquaculture and fisheries in coastal ecosystems represent crucial activities for human feeding. But these biological productions are under the pressure of climate variability and global change. Anticipating the biological processes affected by climate hazards remains a vital objective for species conservation strategies and human activities that rely on. Within marine species, filter feeders like oysters are real key species in coastal ecosystems due to their economic and societal value (fishing and aquaculture) but also due to their ecological importance. Indeed oysters populations in good health play the role of ecosystem engineers that can give many ecosystem services at several scales: building reef habitats that contribute to biodiversity, benthic-pelagic coupling and phytoplankton bloom control through water filtration, living shorelines against coastal erosion… The Pacific oyster, Crassostrea gigas (Thunberg, 1793), which is currently widespread worldwide, was introduced into the Atlantic European coasts at the end of the 19th century for shellfish culture purposes and becomes the main marine species farmed in France (around 100 000 tons) despite severe mortalities crisis. But in the same time and because of warming, natural oysters beds has spread significantly along the French coast and are supposed to have reach approximately 500 000 tons. In that context, Pacific oyster populations (natural and cultivated) in France are the subjects of many scientific projects. Among them, a specific long-term biological monitoring focuses on the reproduction of these populations at a national scale: the VELYGER national program. With more than 8 years of weekly data at many stations in France, this field-monitoring program offers a valuable dataset for studying processes underpinning reproduction cycle of this key-species in relation to environmental parameters, water quality and climate change.   Database content: Larval concentration (number of individuals per 1.5 m3) monitored, since 2008, at several stations in six bays of the French coast (from south to north): Thau Lagoon and bays of Arcachon, Marennes Oléron, Bourgneuf, Vilaine and Brest (see map below).   Methods used to monitor larval concentration: An important volume of seawater (1.5 m3) is pumped twice a week throughout the spawning season (june-september), at one meter below the surface at high tide (+/- 2h) in several sites within each VELYGER ecosystem. Water is filtered trough plankton net fitted with 40 µm mesh. After a proper rinsing of the net, the retained material is transferred into a polyethylene bottle (1 liter) and fixed with alcohol. At laboratory, sample is then gently filtered and rinse again and transferred into eprouvette. Two sub-samples of 1 mL are then taken using a pipette and examined on a graticule slide for microscope. The microscopic examination is made with a conventional binocular optical microscope with micrometer stage at a magnification of 10 X (or above). During the counting, a special care is necessary as larvae of other bivalves are also collected and confusion is possible. Larvae of C. gigas are also classified into four stage of development: - Stage I = D-shaped straight hinge larvae (shell length <105 µm) - Stage II = Early umbo evolved larvae (shell length between 105 and 150 µm) - Stage III = Medium umbo larvae (shell length between 150 and 235 µm) - Stage IV*= Large umbo eyed pediveliger larvae (shell length > 235 µm) * Larvae that are very closed to settle are sometimes identified into a separated 5th stage, but generally this stage is included in stage IV.   Illustrations: Location of the different Velyger sites along the French coast. From south to north: Thau Lagoon and bays of Arcachon, Marennes Oléron, Bourgneuf, Vilaine and Brest.   Legend: Pacific Oyster Larvae (left side) and Natural oyster bed (right side). Photos : © S. Pouvreau/Ifremer

SOMLIT (Service d'Observation en Milieur Littoral) : a French Coastal Monitoring Network Coastal zones are where land, ocean and atmosphere interact. They are important for the exchange of matter and energy, and play a key role in (biogeo)chemical cycles at global scale. These environments are characterised by significant spatial and temporal variability of their physico-chemical and biological parameters due to local and seasonal meteorological drivers which are exacerbated by large-scale climate drivers (e.g. global warming, modification of the wind regime) and local-scale anthropogenic drivers (e.g. nutrient cycle changes linked to the use of fertilisers or the construction of large installations such as dams). These driving mechanisms are often interconnected. In the context of global warming (due to­­ climate and human-induced changes), the identification and understanding of their impact on coastal marine and littoral ecosystems is essential. The scientific objective of SOMLIT is to 1) characterise the multi-decadal evolution of coastal marine and littoral ecosystems, and 2) determine the climatic and anthropogenic drivers. In order to meet this objective, a nationally coordinated multi-site monitoring system was set up in the mid-1990s. The observation strategy is the same for each of the 12 monitored ecosystems with fortnightly sampling and/or measurements, at high tide (for sites subject to tides): 1) in surface-water for a range of 15 parameters (temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonium, phosphate, silicate, suspended particulate matter, chlorophyll a, particulate organic carbon and nitrogen and stable isotopes of particulate organic carbon and nitrogen), 2) in surface-water for a range of 26 parameters of numbering and optical characteristics of pico- and nanoplankton), and 3) along the water column for temperature, salinity, fluorescence and PAR (vertical profiles of multi-parameter probes). SOMLIT’s activities are carried out under a quality assurance / quality control process based on the ISO 17025 standard. SOMLIT’s service provision objectives are to provide data and logistical support for research and other observation activities. SOMLIT has been officially accredited since 1996 as one of the CNRS (French National Centre for Scientific Research) National Observation Services (SNO). SOMLIT’s coordination is hosted by the Observatoire Aquitain des Sciences de l'Univers (University of Bordeaux / CNRS) and the service relies on strong partnerships with nine other institutions (University of Lille, University of the Littoral Opal Coast, University of Caen Normandy, Sorbonne University, University of Western Brittany, La Rochelle University, University of Montpellier, Aix Marseille University, National Museum of Natural History). SOMLIT is one of the nine networks that compose France’s Coastal Research Infrastructure (ILICO).  SOMLIT has strong ties with ILICO’s other networks such as the SNOs MOOSE (Mediterranean Ocean Observing System on Environment), PHYTOBS (microphytoplankton monitoring) and COAST-HF (Coastal Ocean Observing System - High Frequency).

LOCEAN has been in charge of analyzing the isotopic composition of the dissolved inorganic carbon (DIC) in sea water collected during a series of cruises or ships of opportunity mostly in the southern Indian Ocean , the North Atlantic, and the equatorial Atlantic, but also in the Mediterranean Sea and in the equatorial Pacific. The LOCEAN sea-water samples for δ13CDIC were collected in 125/25 ml glass bottles until 2022/since then and poisoned with HgCl2 (1 ml of saturated solution) before storage in a dark room à 4°C until their measurement. The DIC was extracted from the seawater by acidification with phosphoric acid (H3PO4 85%) and CO2 gas that was produced was collected in a vacuum system following the procedure described by Kroopnick (1974). The isotopic composition of CO2 was determined using a dual inlet-isotopic ratio mass spectrometer (SIRA9-VG) by comparing the 13C/12C ratio of the sample to the 13C/12C ratio of a reference material, the Vienna-Pee Dee Belemnite (V-PDB). The isotopic composition is expressed in the δ-unit defined by Craig (1957)(method type 2).  Experience showed that samples older than 3-4 years are likely to have experienced conservation issues and have been dismissed. The mass spectrometer has worked very well until 2014-2015. Afterwards, its aging as well as the aging of the preparation line resulted in more data loss, and often less accurate results. The preparation line was renovated in 2019, and analyses in 2020 were run manually, often repeating the measurement a second time for each sample. Up to 2007-2008, δ13CDIC values have a precision of±0.01 ‰ (Vangriesheim et al.,2009) and a reproducibility of±0.02 ‰. After an interlaboratory comparison exercise led by Claire Normandeau (Dalhousie  University),  results  suggest  that  recent  LOCEAN  samples have a slightly poorer reproducibility (±0.04 ‰ ) as well as an offset of -0.13‰ (details available in Reverdin et al., ESSD 2018) that is confirmed by Becker et al. 2016 work by comparison with other cruises after removing the anthropogenic signal. Recent comparisons in early May 2021 with Orsay GEOPS facility samples suggest that the current offset is much smaller and might be +0.03‰. LOCEAN has installed in 2021 a new measurement device by coupling a Picarro G2131-I cavity ring down spectrometer (CRDS) with a CO2 extractor (Apollo SciTech) that will measure at the same time DIC (method type 3) (Leseurre, 2022). Since then, all water samples have been analyzed on this device. Part of the data set, as well as a scientific context and publications are also presented on the WEB site https://www.locean-ipsl.upmc.fr/oceans13c. Individual files correspond to regional subsets of the whole dataset. The file names are based on two letters for the region followed by (-) the cruise or project name (see below) followed by –DICisotopes, followed by either -s (surface data) or -b (subsurface data), and a version number (-V0, …): example SI-OISO-DICisotopes-s-V0; the highest version number corresponds to the latest update of the cruise/project data set, and can be directly downloaded. Earlier versions can be obtained on request, but are not recommended. The region two letters are the followings:   - SI: station and surface data in the Southern Indian Ocean that include cruises : INDIGO I (1985 – stn) (https://doi.org/10.17600/85000111) CIVA I (1993 – stn & surf) (https://doi.org/10.17600/93000870) (Archambeau et al., JMS 1998) ANTARES (1993 – stn & surf) (https://doi.org/10.17600/93000600) OISO (*) (since 1998 – stn & surf) (https://doi.org/10.18142/228) (Racapé et al., Tellus 2010, Leseurre, 2022)   - EA: station and surface data in the Tropical Atlantic Ocean that include cruises : EQUALANT (1999 & 2000 – surf) (https://doi.org/10.18142/98) EGEE (2005 to 2007 – stn & surf) (https://doi.org/10.18142/95) PIRATA (since 2013 – stn & surf) (https://doi.org/10.18142/14) EUMELI 2 (1991 – stn) (https://doi.org/10.17600/91004011)  (Pierre et al., JMS 1994) BIOZAIRE 3 (2003 – stn & surf ) (https://doi.org/10.17600/3010120) (Vangriesheim et al., DSRII, 2009) TARA-Microbiomes (2021 - stn & surf)   - NA : station and surface data in the North Atlantic Subpolar gyre that include cruises : OVIDE (**) (since 2002 – stn & surf) (https://doi.org/10.17882/46448) (Racapé et al., 2013) RREX (2017 – stn & surf) (https://doi.org/10.17600/17001400) SURATLANT (since 2010 - surf) (https://doi.org/10.17882/54517) (Racapé et al., BG 2014 ; Reverdin et al., ESSD 2018, Leseurre, 2022) NUKATUKUMA (since 2017- surf)   - MS: station data in the Mediterranean sea that include cruises : ALMOFRONT 1 (1991 – stn) (https://doi.org/10.17600/91004211) VICOMED 3 (1990 – stn) (https://doi.org/10.17600/90000711)   - PO: tropical Pacific that include cruises : PANDORA (2012 – stn) (https://doi.org/10.17600/12010050) ALIZE2 (1991 – stn & surf) (https://doi.org/10.17600/91002711) (Laube-Lenfant and Pierre, Oceanologica Acta 1994)   - SO: station and surface data in the Southern Ocean (except OISO) that include cruises: TARA-Microbiomes (2021-2022, stn & surf) AGULHASII-072022 (2022, stn) CONFLUENCE (1993-1994, stn)   - AO: station and surface data in the Arctic Ocean and nearby seas that include cruises: GREENFEEDBACK (2024, stn&surf) TCA (2024, stn) REFUGE ARCTIC (2024, stn) (*) The values for cruises OISO19, 21 and 22 are doubtful (for some, too low) and will require further investigation to find whether adjusted values can be proposed. (**) Some of the OVIDE cruises are also referred to as or GEOVIDE (in 2014), and BOCATS (in 2016). CATARINA, BOCATS1 and BOCATS2 (PID2019-104279GB-C21/AEI/10.13039/501100011033) cruises were funded by the Spanish Research Agency  The values of the OVIDE 2010 stations are doubtful (too low), but no particular error was found, and they have been left in the files.   Data The files are in csv format reported as: - Cruise name, station id, (bottle number), day, month, year, hour, minute, longitude, latitude, pressure (db), depth (m), temperature (°C), temperature qc, salinity (pss-78), salinity qc, d13CDIC, d13CDIC qc, method type - Temperature is an in situ temperature - Salinity is a practical salinity - Method type (1) acid CO2 extraction from helium stripping technique coupled to mass spectrometer, (2) acid CO2 extraction in a vacuum system coupled to mass spectrometer,(3) CO2 extractor (Apollo SciTech) coupled to CRDS measurements. Temperature qc, salinity qc, d13CDIC qc are quality indices equal to: - 0 no quality check (but presumably good data) - 1 probably good data - 2 good data - 3 probably bad data - 4 certainly bad data - 9 missing data (and the missing data are reported with an unlikely missing value)